Clostridia are anaerobic Gram-positive Firmicutes containing broad and flexible systems for substrate utilization, which have been used successfully to produce a range of industrial compounds.
In particular, Clostridium acetobutylicum has been used to produce butanol on an industrial scale through acetone-butanol-ethanol (ABE) fermentation.
We have developed a metabolic model for Clostridium acetobutylicum, iCac802 that spans 802 genes and includes 1,137 metabolites and 1,462 reactions, along with gene-protein-reaction associations. All reactions present are elementally and charge balanced. GPR associations were determined from the available functional annotation information and homology predictions accounting for monofunctional proteins, multifunctional proteins, isozymes, and protein complexes. The model was curated to remove any thermodynamically infeasible cycles, as detailed in the Methods section.
Dash, S., Mueller, T. J., Venkataramanan, K. P., Papoutsakis, E. T., & Maranas, C. D. (2014). "Capturing the response of Clostridium acetobutylicum to chemical stressors using a regulated genome-scale metabolic model". Biotechnology for Biofuels, 7(1), 144.
The model is licensed under a Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Before downloading our model, we ask for your name and institution so we may better design future versions of our software. Every consideration has been made during the design, writing, and posting of this model to ensure it works properly.